Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis.The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation.However, suberin aliphatic components are isolated together with all the other -N CHAUSSURES acyl chains making up the lipids (e.
g., membranes) present in roots.For the solvent-extraction method, roots are first delipidated before transmethylation.
This method is longer but allows separation Internal Hard Drives of soluble and polymerized root lipids.This protocol is optimized for tissue culture- or soil-grown Arabidopsis thaliana plants, but can be used with roots of other plants.